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The enzymic activity of Glucanase is effective in the temperature range from 40°C to 75°C, with the optimum performance at 60°C.
The pH range for the activity of Glucanase is approximately from 3.5 – 6.5, with an optimum performance at pH 5.5. The exact pH optimum will depend on process variables, including temperature, time, substrate nature and concentration.
Brewhouse
Glucanase should be added directly to the mash at between 0.5kg and 1kg/tonne of grist, depending upon the level of β-glucans present and the composition of the mash.
Fermentation
In a fermentation vessel, Glucanase can be used at a rate between 7 and 20ml/hl, dependent upon the gravity of the beer and amount of glucan.
Conditioning
In a conditioning tank, Glucanase can be used at a rate between 7.5 and 20ml/hl.
Malting
Glucanase should be diluted into the chitting water and sprayed onto the malt at a rate of between 0.05% and 0.2% w/w on barley; or between 0.5 and 2.5 ml/kg barley in the normal volume of chitting water.
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